See production charts
See modification tables
Batch
Titer
1.35 g/L (
Escherichia coli DH5α
)
1.52 g/L (
Escherichia coli YF-3
)
2.03 g/L (
Escherichia coli YF-4
)
2.29 g/L (
Escherichia coli YF-5
)
2.51 g/L (
Escherichia coli YF-6
)
4.5 g/L (
Escherichia coli YF-7
)
3.7 g/L (
Escherichia coli YF-8
)
Fed-Batch
Titer
8.34 g/L (
Escherichia coli YF-7
)
8.3 g/L (
Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
)
6.69 g/L (
Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His)
)
9.08 g/L (
Escherichia coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
)
3.7 g/L (
Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
)
9.0 g/L (
Escherichia coli Q1 (DE3) thrA S357R (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
)
37.3 g/L (
Escherichia coli ALE-5
)
34.8 g/L (
Escherichia coli ES-134/pSC-08
)
23.8 g/L (
Escherichia coli ES-1/pSC-08
)
29.6 g/L (
Escherichia coli ES-13/pSC-08
)
26.3 g/L (
Escherichia coli ES-1234/pSC-08
)
Volumetric productivity
0.18 g/L/h (
Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
)
0.13 g/L/h (
Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His)
)
0.18 g/L/h (
Escherichia coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
)
0.83 g/L/h (
Escherichia coli ALE-5
)
Substrate yield
0.12 g/g of sustrate (
Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
)
0.1 g/g of sustrate (
Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His)
)
0.18 g/g of sustrate (
Escherichia coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
)
0.24 g/g of sustrate (
Escherichia coli ALE-5
)
0.32 g/g of sustrate (
Escherichia coli ES-134/pSC-08
)
0.25 g/g of sustrate (
Escherichia coli ES-1/pSC-08
)
0.31 g/g of sustrate (
Escherichia coli ES-13/pSC-08
)
0.29 g/g of sustrate (
Escherichia coli ES-1234/pSC-08
)
Flask
Titer
3.8 g/L (
Escherichia coli (pBCBA, pJD760)
)
0.49 g/L (
Escherichia coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB)
)
0.53 g/L (
Escherichia coli T1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
)
0.89 g/L (
Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
)
266.3 mg/L (
Escherichia coli E4G2 (pPK10)
)
83.3 mg/L (
Escherichia coli E3 (pPK10)
)
137.1 mg/L (
Escherichia coli E4G1 (pPK10)
)
129.0 mg/L (
Escherichia coli ES/pSC-08
)
384.0 mg/L (
Escherichia coli ES-1234/pSC-08
)
220.0 mg/L (
Escherichia coli ES-1/pSC-08
)
385.0 mg/L (
Escherichia coli ES-13/pSC-08
)
445.0 mg/L (
Escherichia coli ES-134/pSC-08
)
Substrate yield
0.24 g/g of sustrate (
Escherichia coli (pBCBA, pJD760)
)
0.245 g/g of sustrate (
Escherichia coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB)
)
Biomass-specific titer
0.73 g/L/g CDW (
Escherichia coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB)
)
1.94 g/L/g CDW (
Escherichia coli T1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
)
Volumetric productivity
2.73 g/L/h (
Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
)