Titer in Flask for L-serine

Strain Production
mg/L
1000
2000
3000
4000
5000
E. coli E3 (pPK10)
E. coli ES/pSC-08
E. coli E4G1 (pPK10)
E. coli ES-1/pSC-08
E. coli E4G2 (pPK10)
E. coli ES-1234/pSC-08
E. coli ES-13/pSC-08
E. coli ES-134/pSC-08
E. coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB)
E. coli T1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
E. coli DH5α
E. coli YF-3
E. coli YF-4
E. coli YF-5
E. coli YF-6
E. coli YF-8
E. coli (pBCBA, pJD760)
E. coli YF-7

Titer in Fed-Batch for L-serine

Strain Production
mg/L
8000.0
16000.0
24000.0
32000.0
40000.0
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His)
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
E. coli YF-7
E. coli Q1 (DE3) thrA S357R (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
E. coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
E. coli ES-1/pSC-08
E. coli ES-1234/pSC-08
E. coli ES-13/pSC-08
E. coli ES-134/pSC-08
E. coli ALE-5

Substrate yield in Flask for L-serine

Strain Production
g/g of sustrate
0.05
0.1
0.15
0.2
0.25
0.3
E. coli (pBCBA, pJD760)
E. coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB)

Substrate yield in Fed-Batch for L-serine

Strain Production
g/g of sustrate
0.08
0.16
0.24
0.32
0.4
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His)
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
E. coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
E. coli ALE-5
E. coli ES-1/pSC-08
E. coli ES-1234/pSC-08
E. coli ES-13/pSC-08
E. coli ES-134/pSC-08

Biomass-specific productivity in Flask for L-serine

Strain Production
mg/L/g DCW
400.0
800.0
1200.0
1600.0
2000.0
E. coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB)
E. coli T1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)

Volumetric productivity in Flask for L-serine

Strain Production
mg/L/h
500.0
1000.0
1500.0
2000.0
2500.0
3000.0
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)

Volumetric productivity in Fed-Batch for L-serine

Strain Production
mg/L/h
100
200
300
400
500
600
700
800
900
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His)
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
E. coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
E. coli ALE-5