Titer in Flask for L-serine

Modifications
Strain Maximum production sdaA iclR arcA serA serB serC eamA sdaB tdcG glyA gcvH gcvP gcvT kbl tdh pgk serA sdaC cycA sstT tdcC
E. coli E3 (pPK10) 83.3 mg/L
E. coli ES/pSC-08 129 mg/L
E. coli E4G1 (pPK10) 137.1 mg/L
E. coli ES-1/pSC-08 220 mg/L
E. coli E4G2 (pPK10) 266.3 mg/L
E. coli ES-1234/pSC-08 384 mg/L
E. coli ES-13/pSC-08 385 mg/L
E. coli ES-134/pSC-08 445 mg/L
E. coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB) 490 mg/L
E. coli T1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 530 mg/L
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 890 mg/L
E. coli DH5α 1,350 mg/L
E. coli YF-3 1,520 mg/L
E. coli YF-4 2,030 mg/L
E. coli YF-5 2,290 mg/L
E. coli YF-6 2,510 mg/L
E. coli YF-8 3,700 mg/L
E. coli (pBCBA, pJD760) 3,800 mg/L
E. coli YF-7 4,500 mg/L

Titer in Fed-Batch for L-serine

Modifications
Strain Maximum production sdaA iclR arcA serA serB serC sdaB tdcG glyA eamA rhtA ompX opgE rybA mntS thrA sdaC sstT tdcC pgk cycA
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His) 6,690 mg/L
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 8,300 mg/L
E. coli YF-7 8,340 mg/L
E. coli Q1 (DE3) thrA S357R (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 9,000 mg/L
E. coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 9,080 mg/L
E. coli ES-1/pSC-08 23,800 mg/L
E. coli ES-1234/pSC-08 26,300 mg/L
E. coli ES-13/pSC-08 29,600 mg/L
E. coli ES-134/pSC-08 34,800 mg/L
E. coli ALE-5 37,300 mg/L

Substrate yield in Flask for L-serine

Modifications
Strain Maximum production serC serB eamA sdaA serA
E. coli (pBCBA, pJD760) 0.24 g/g of sustrate
E. coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB) 0.245 g/g of sustrate

Substrate yield in Fed-Batch for L-serine

Modifications
Strain Maximum production sdaA sdaB tdcG glyA serA serC serB eamA rhtA ompX opgE rybA mntS sdaC sstT tdcC pgk cycA
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His) 0.1 g/g of sustrate
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 0.12 g/g of sustrate
E. coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 0.18 g/g of sustrate
E. coli ALE-5 0.24 g/g of sustrate
E. coli ES-1/pSC-08 0.25 g/g of sustrate
E. coli ES-1234/pSC-08 0.29 g/g of sustrate
E. coli ES-13/pSC-08 0.31 g/g of sustrate
E. coli ES-134/pSC-08 0.32 g/g of sustrate

Biomass-specific productivity in Flask for L-serine

Modifications
Strain Maximum production sdaA serA serC serB sdaB tdcG
E. coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB) 730 mg/L/g DCW
E. coli T1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 1,940 mg/L/g DCW

Volumetric productivity in Flask for L-serine

Modifications
Strain Maximum production sdaA sdaB tdcG glyA serA serC serB
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 2,730 mg/L/h

Volumetric productivity in Fed-Batch for L-serine

Modifications
Strain Maximum production sdaA sdaB tdcG glyA serA serC serB eamA rhtA ompX opgE rybA mntS
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His) 130 mg/L/h
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 180 mg/L/h
E. coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 180 mg/L/h
E. coli ALE-5 830 mg/L/h