Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications

Titer in Batch for L-serine

Modifications
Strain Maximum production sdaAEc iclREc arcAEc serAEc serBEc serCEc
E. coli DH5α 1,350 mg/L
E. coli YF-3 1,520 mg/L
E. coli YF-4 2,030 mg/L
E. coli YF-5 2,290 mg/L
E. coli YF-6 2,510 mg/L
E. coli YF-8 3,700 mg/L
E. coli YF-7 4,500 mg/L

Titer in Fed-Batch for L-serine

Modifications
Strain Maximum production sdaAEc iclREc arcAEc serAEc serBEc serCEc sdaBEc tdcGEc glyAEc eamAEc rhtAEc ompXEc opgEEc rybAEc mntSEc thrAEc sdaCEc sstTEc tdcCEc pgkEc cycAEc
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His) 6,690 mg/L
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 8,300 mg/L
E. coli YF-7 8,340 mg/L
E. coli Q1 (DE3) thrA S357R (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 9,000 mg/L
E. coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 9,080 mg/L
E. coli ES-1/pSC-08 23,800 mg/L
E. coli ES-1234/pSC-08 26,300 mg/L
E. coli ES-13/pSC-08 29,600 mg/L
E. coli ES-134/pSC-08 34,800 mg/L
E. coli ALE-5 37,300 mg/L

Titer in Flask for L-serine

Modifications
Strain Maximum production serCEc serBEc eamAEc sdaAEc serAEc sdaBEc tdcGEc glyAEc gcvHEc gcvPEc gcvTEc kblEc tdhEc pgkEc serACg sdaCEc cycAEc sstTEc tdcCEc
E. coli E3 (pPK10) 83.3 mg/L
E. coli ES/pSC-08 129 mg/L
E. coli E4G1 (pPK10) 137.1 mg/L
E. coli ES-1/pSC-08 220 mg/L
E. coli E4G2 (pPK10) 266.3 mg/L
E. coli ES-1234/pSC-08 384 mg/L
E. coli ES-13/pSC-08 385 mg/L
E. coli ES-134/pSC-08 445 mg/L
E. coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB) 490 mg/L
E. coli T1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 530 mg/L
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 890 mg/L
E. coli (pBCBA, pJD760) 3,800 mg/L

Substrate yield in Flask for L-serine

Modifications
Strain Maximum production serCEc serBEc eamAEc sdaAEc serAEc
E. coli (pBCBA, pJD760) 0.24 g/g of sustrate
E. coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB) 0.245 g/g of sustrate

Substrate yield in Fed-Batch for L-serine

Modifications
Strain Maximum production sdaAEc sdaBEc tdcGEc glyAEc serAEc serCEc serBEc eamAEc rhtAEc ompXEc opgEEc rybAEc mntSEc sdaCEc sstTEc tdcCEc pgkEc cycAEc
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His) 0.1 g/g of sustrate
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 0.12 g/g of sustrate
E. coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 0.18 g/g of sustrate
E. coli ALE-5 0.24 g/g of sustrate
E. coli ES-1/pSC-08 0.25 g/g of sustrate
E. coli ES-1234/pSC-08 0.29 g/g of sustrate
E. coli ES-13/pSC-08 0.31 g/g of sustrate
E. coli ES-134/pSC-08 0.32 g/g of sustrate

Biomass-specific titer in Flask for L-serine

Modifications
Strain Maximum production sdaAEc serAEc serCEc serBEc sdaBEc tdcGEc
E. coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB) 730
E. coli T1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 1,940

Volumetric productivity in Flask for L-serine

Modifications
Strain Maximum production sdaAEc sdaBEc tdcGEc glyAEc serAEc serCEc serBEc
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 2,730 mg/L/h

Volumetric productivity in Fed-Batch for L-serine

Modifications
Strain Maximum production sdaAEc sdaBEc tdcGEc glyAEc serAEc serCEc serBEc eamAEc rhtAEc ompXEc opgEEc rybAEc mntSEc
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His) 130 mg/L/h
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 180 mg/L/h
E. coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) 180 mg/L/h
E. coli ALE-5 830 mg/L/h