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Han Liu, Guochen Fang, Hui Wu, Zhimin Li & Qin Ye (2018). L‐Cysteine Production in Escherichia coli Based on Rational Metabolic Engineering and Modular Strategy. Biotechnology Journal.

Strains
  • Escherichia coli SLH01
  • Escherichia coli LH00
  • Escherichia coli LH01
  • Escherichia coli LH02
  • Escherichia coli LH03
  • Escherichia coli LH04
  • Escherichia coli LH05
  • Escherichia coli SLH02
  • Escherichia coli LH06
  • Escherichia coli JM109

Plasmids
  • pLH01
  • pLH02
  • pTrcA
  • pTrcAC
  • pTrcACB
  • pLH03
  • pACYC184

Metabolite production
  • acetate
      Titer
    • 3.29 g/L (Escherichia coli LH03 in Flask)
  • L-cysteine
      Titer
    • 5.1 g/L (Escherichia coli LH06 in Fed-Batch)
    • 620.9 mg/L (Escherichia coli LH06 in Flask)
    • 547.3 mg/L (Escherichia coli LH05 in Flask)
    • 45.8 mg/L (Escherichia coli LH03 in Flask)
    • 190.3 mg/L (Escherichia coli LH03 in Flask)
    • 492.0 mg/L (Escherichia coli LH04 in Flask)
    • Substrate yield
    • 0.06 g/g of sustrate (Escherichia coli LH06 in Flask)
    • 0.053 g/g of sustrate (Escherichia coli LH05 in Flask)
    • 0.004 g/g of sustrate (Escherichia coli LH03 in Flask)
    • 0.018 g/g of sustrate (Escherichia coli LH03 in Flask)
    • 0.047 g/g of sustrate (Escherichia coli LH04 in Flask)

Reference

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