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Gen Nonaka & Kazuhiro Takumi (2017). Cysteine degradation gene yhaM, encoding cysteine desulfidase, serves as a genetic engineering target to improve cysteine production in Escherichia coli. AMB Express.

Strains
  • Escherichia coli MG1655 (pMIV-cysE5, pACYC177)
  • Escherichia coli MG1655 (pMIV-cysE5, pACYC-yhaM1)
  • Escherichia coli MG1655 (pMIV-cysE5)
  • Escherichia coli MG1655 ΔyhaM
  • Escherichia coli MG1655 ΔyhaM (pMIV-cysE5)

Plasmids
  • pSTV29
  • pSTV-ydeD5
  • pSTV-yhaM7
  • pACYC177
  • pACYC-yhaM1
  • pMIV-cysE5

Metabolite production
  • L-cysteine
      Titer
    • 74.6 mg/L (Escherichia coli MG1655 (pMIV-cysE5, pACYC177) in Flask)
    • 14.9 mg/L (Escherichia coli MG1655 (pMIV-cysE5, pACYC-yhaM1) in Flask)
    • 316.2 mg/L (Escherichia coli MG1655 ΔyhaM (pMIV-cysE5) in Flask)
    • 64.6 mg/L (Escherichia coli MG1655 (pMIV-cysE5) in Flask)
    • Substrate yield
    • 0.187 % (Escherichia coli MG1655 (pMIV-cysE5, pACYC177) in Flask)
    • 0.037 % (Escherichia coli MG1655 (pMIV-cysE5, pACYC-yhaM1) in Flask)
    • 0.81 % (Escherichia coli MG1655 ΔyhaM (pMIV-cysE5) in Flask)
    • 0.164 % (Escherichia coli MG1655 (pMIV-cysE5) in Flask)

Reference

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