Zheng, Zhaojuan; Zhao, Mingyue; Zang, Ying; Zhou, Ying & Ouyang, Jia. Production of optically pure l-phenyllactic acid by using engineered Escherichia coli coexpressing l-lactate dehydrogenase and formate dehydrogenase. Journal of Biotechnology. 2015, 207, 47-51.

Strains

• Escherichia coli BL21(pETDuet-ldhL-fdh)
• Escherichia coli BL21(DE3)

Plasmids

• pEASY-Blunt
• pETDuet-ldhL-fdh
• pETDuet-fdh
• pETDuet-1

Metabolite production

• acetate
  Titer
  • 1.98 g/L (Escherichia coli MG-P10 in Fed-Batch)
• L-(-)-3-Phenyllactic acid
  Titer
  • 79.6 mM (Escherichia coli BL21(pETDuet-ldhL-fdh) in Flask)
  • 119.4 mM/h (Escherichia coli BL21(pETDuet-ldhL-fdh) in Flask)
  Substrate yield
  • 96.1 % (Escherichia coli BL21(pETDuet-ldhL-fdh) in Flask)