Li, Hua; Wang, Baoshi; Zhu, Linghuan; Cheng, Shi; Li, Youran; Zhang, Liang; Ding, Zhong Yang; Gu, Zheng Hua & Shi, Gui Yang. Metabolic engineering of Escherichia coli W3110 for L-homoserine production. Process Biochemistry. 2016, 51(12), 1973-1983.
Strains
- Escherichia coli HM1
- Escherichia coli HM2
- Escherichia coli HM3
- Escherichia coli HM4
- Escherichia coli HM5
- Escherichia coli HM6
- Escherichia coli HM4 (pBRlysC)
- Escherichia coli HM4 (pBRlysCFbr)
- Escherichia coli HM4 (pBRthrA)
- Escherichia coli HM4 (pBRthrAFbr)
- Escherichia coli HM4 (pBRmetL)
- Escherichia coli HM3 (pBRmetL)
- Escherichia coli HM4 (pBRmetL–rhtA)
- Escherichia coli HM4 (pBRmetL–rhtA23)
- Escherichia coli HM4 (pBRmetL–pNrhtA)
- Escherichia coli HM5 (pBRmetL–pNrhtA)
- Escherichia coli W3110
Plasmids
- pMD18-T
- pKK223-3
- pBR322
- pETthrA
- pETthrAFbr
- pETmetL
- pETlysC
- pBRthrA
- pBRthrAFbr
- pBRmetL
- pBRlysC
- pBRlysCFbr
- pKKtdcC
- pBRmetL–pNrhtA
- pKKpNrhtA
- pBRmetL–rhtA
- pBRmetL–rhtA23
- pET-28a(+)
Metabolite production
-
L-homoserine
-
Titer
- 0.12 g/L (Escherichia coli HM4 in Flask)
- 0.0 g/L (Escherichia coli W3110 in Flask)
- 1.4 g/L (Escherichia coli HM3 (pBRmetL) in Fed-Batch)
- 0.4 g/L (Escherichia coli HM4 (pBRlysC) in Flask)
- 0.35 g/L (Escherichia coli HM4 (pBRlysCFbr) in Flask)
- 1.2 g/L (Escherichia coli HM4 (pBRthrA) in Flask)
- 1.11 g/L (Escherichia coli HM4 (pBRthrAFbr) in Flask)
- 1.04 g/L (Escherichia coli HM4 (pBRmetL) in Flask)
- 6.78 g/L (Escherichia coli HM4 (pBRmetL) in Fed-Batch)
- 2.12 g/L (Escherichia coli HM4 (pBRmetL–pNrhtA) in Flask)
- 1.81 g/L (Escherichia coli HM4 (pBRmetL–rhtA23) in Flask)
- 1.42 g/L (Escherichia coli HM4 (pBRmetL–rhtA) in Flask)
- 33.42 g/L (Escherichia coli HM4 (pBRmetL–pNrhtA) in Flask)
- 39.54 g/L (Escherichia coli HM5 (pBRmetL–pNrhtA) in Fed-Batch)
- 0.29 g/g of sustrate (Escherichia coli HM5 (pBRmetL–pNrhtA) in Flask)
- 0.9 g/L/h (Escherichia coli HM5 (pBRmetL–pNrhtA) in Fed-Batch)
- 33.42 g/L (Escherichia coli HM4 (pBRmetL–pNrhtA) in Fed-Batch)
- 6.78 g/L (Escherichia coli HM4 (pBRmetL) in Fed-Batch) Substrate yield
- 0.26 g/g of sustrate (Escherichia coli HM4 (pBRmetL–pNrhtA) in Flask)
- 0.24 g/g of sustrate (Escherichia coli HM4 (pBRmetL–pNrhtA) in Fed-Batch)
- 0.13 g/g of sustrate (Escherichia coli HM4 (pBRmetL) in Fed-Batch)
-
L-threonine
-
Titer
- 20.62 g/L (Escherichia coli HM3 (pBRmetL) in Fed-Batch)
-
acetate
-
Titer
- 3.54 g/L (Escherichia coli HM4 (pBRmetL–pNrhtA) in Fed-Batch)
- 1.3 g/L (Escherichia coli HM4 (pBRmetL) in Fed-Batch)
- 5.2 g/L (Escherichia coli HM5 (pBRmetL–pNrhtA) in Fed-Batch) Substrate yield
- 0.025 g/g of sustrate (Escherichia coli HM4 (pBRmetL) in Fed-Batch)
- 0.027 g/g of sustrate (Escherichia coli HM4 (pBRmetL–pNrhtA) in Fed-Batch)
- 0.038 g/L (Escherichia coli HM5 (pBRmetL–pNrhtA) in Fed-Batch)