Wu, Weibin; Chen, Maosen; Li, Chenxi; Zhong, Jie; Xie, Rusheng; Pan, Zhibin; Lin, Junhan & Qi, Feng. Efficient production of phenyllactic acid in Escherichia coli via metabolic engineering and fermentation optimization strategies. Frontiers in Microbiology. 2024, 15.
Strains
- Escherichia coli CGSC4510
- Escherichia coli MG-P4
- Escherichia coli MP-P5
- Escherichia coli MG-P6
- Escherichia coli MG-P10
- Escherichia coli MG-P1
- Escherichia coli MG-P2
- Escherichia coli MG-P3
- Escherichia coli MG-P7
- Escherichia coli MG-P8
- Escherichia coli MG-P9
Plasmids
Metabolite production
-
acetate
-
Titer
- 0.81 g/L (Escherichia coli MG-P10 in Fed-Batch)
- 4.5 g/L (Escherichia coli MG-P10 in Fed-Batch)
- 2.68 g/L (Escherichia coli MG-P10 in Fed-Batch)
-
L-(-)-3-Phenyllactic acid
-
Titer
- 0.028 g/L (Escherichia coli CGSC4510 in Flask)
- 0.251 g/L (Escherichia coli MG-P1 in Flask)
- 0.189 g/L (Escherichia coli MG-P2 in Flask)
- 0.212 g/L (Escherichia coli MG-P3 in Flask)
- 0.417 g/L (Escherichia coli MG-P4 in Flask)
- 0.578 g/L (Escherichia coli MP-P5 in Flask)
- 0.7174 g/L (Escherichia coli MG-P6 in Flask)
- 1.14 g/L (Escherichia coli MG-P8 in Flask)
- 1.21 g/L (Escherichia coli MG-P9 in Flask)
- 1.42 g/L (Escherichia coli MG-P10 in Flask)
- 52.89 g/L (Escherichia coli MG-P10 in Fed-Batch)
- 45.68 g/L (Escherichia coli MG-P10 in Fed-Batch)
- 31.01 g/L (Escherichia coli MG-P10 in Fed-Batch)
- 49.68 g/L (Escherichia coli MG-P10 in Fed-Batch)
- 0.88 g/L (Escherichia coli MG-P7 in Flask) Substrate yield
- 0.177 g/g of sustrate (Escherichia coli MG-P10 in Fed-Batch)
- 0.225 g/g of sustrate (Escherichia coli MG-P10 in Fed-Batch)
- 0.181 g/g of sustrate (Escherichia coli MG-P10 in Fed-Batch)
- 0.205 g/g of sustrate (Escherichia coli MG-P10 in Fed-Batch) Volumetric productivity
- 0.646 g/L/h (Escherichia coli MG-P10 in Fed-Batch)
- 1.102 g/L/h (Escherichia coli MG-P10 in Fed-Batch)
- 0.951 g/L/h (Escherichia coli MG-P10 in Fed-Batch)
- 1.035 g/L/h (Escherichia coli MG-P10 in Fed-Batch)