Hao Zhang, Zhong Liang, Ming Zhao, Yanqin Ma, Zhengshan Luo, Sha Li & Hong Xu (2022). Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor. Frontiers in Bioengineering and Biotechnology.

Strains

• Escherichia coli ET00
• Escherichia coli ET01
• Escherichia coli ET02
• Escherichia coli ET03
• Escherichia coli ET04
• Escherichia coli ET05
• Escherichia coli ET06
• Escherichia coli ET07
• Escherichia coli ET08
• Escherichia coli ET08-N
• Escherichia coli ET09
• Escherichia coli ET10
• Escherichia coli ET11
• Escherichia coli ET12
• Escherichia coli ET13
• Escherichia coli ET14
• Escherichia coli ET15
• Escherichia coli BL21(DE3)
• Escherichia coli MG1655

Plasmids

• pET-28a(+)
• pTrc-ectABC
• pET-ectABC
• pTETA
• pTETB
• pTETC
• pTETATA
• pTETBTB
• pTETCTC
• pTETBTC
• pTrc99A

Metabolite production

• ectoine
  Titer
  • 0.0 g/L (Escherichia coli ET00 in Flask)
  • 0.52 g/L (Escherichia coli ET01 in Flask)
  • 0.0 g/L (Escherichia coli ET02 in Flask)
  • 1.09 g/L (Escherichia coli ET06 in Flask)
  • 1.87 g/L (Escherichia coli ET08 in Flask)
  • 1.08 g/L (Escherichia coli ET03 in Flask)
  • 0.34 g/L (Escherichia coli ET04 in Flask)
  • 0.22 g/L (Escherichia coli ET05 in Flask)
  • 0.61 g/L (Escherichia coli ET07 in Flask)
  • 7.29 g/L (Escherichia coli ET08 in Flask)
  • 6.18 g/L (Escherichia coli ET08 in Flask)
  • 10.2 g/L (Escherichia coli ET08 in Flask)
  • 6.85 g/L (Escherichia coli ET09 in Flask)
  • 2.39 g/L (Escherichia coli ET10 in Flask)
  • 12.9 g/L (Escherichia coli ET11 in Flask)
  • 7.42 g/L (Escherichia coli ET15 in Flask)
  • 4.38 g/L (Escherichia coli ET12 in Flask)
  • 9.05 g/L (Escherichia coli ET13 in Flask)
  • 1.04 g/L (Escherichia coli ET14 in Flask)
  • 53.2 g/L (Escherichia coli ET11 in Fed-Batch)
  Biomass-specific yield
  • 0.62 g/g DCW (Escherichia coli ET13 in Flask)
  • 0.59 g/g DCW (Escherichia coli ET08 in Flask)
  • 0.41 g/g DCW (Escherichia coli ET15 in Flask)
  • 0.74 g/g DCW (Escherichia coli ET11 in Flask)
  • 2.0 g/g DCW (Escherichia coli ET11 in Fed-Batch)
  Volumetric productivity
  • 1.11 g/L/h (Escherichia coli ET11 in Fed-Batch)
  Substrate yield
  • 0.33 g/g of sustrate (Escherichia coli ET11 in Fed-Batch)
• acetate
  Titer
  • 6.52 g/L (Escherichia coli ET03 in Flask)
  • 1.81 g/L (Escherichia coli ET06 in Flask)
  • 3.25 g/L (Escherichia coli ET08 in Flask)