Lin, Zhenquan; Xu, Zhibo; Li, Yifan; Wang, Zhiwen; Chen, Tao & Zhao, Xueming. Metabolic engineering of Escherichia coli for the production of riboflavin. Microbial Cell Factories. 2014, 13(1).

Strains

• Escherichia coli RF01S
• Escherichia coli RF02
• Escherichia coli RF02S
• Escherichia coli RF03S
• Escherichia coli RF05
• Escherichia coli RF05S
• Escherichia coli MG1655/p15C-EC10
• Escherichia coli MG1655/p5C-EC10
• Escherichia coli MG1655/p20C-BSrib
• Escherichia coli MG1655/p20C-BS15
• Escherichia coli RF01S/p15Trc-zg
• Escherichia coli RF01S/p15Trc
• Escherichia coli RF01S/p15Trc-zgp
• Escherichia coli RF03
• Escherichia coli MG1655

Plasmids

• p15C-EC10
• p5C
• p5C-EC10
• p20C-EC10
• p20C-BS15
• p20C-BSrib
• p20C
• p15Trc
• p15Trc-zg
• p15Trc-zgp

Metabolite production

• riboflavin
  Titer
  • 229.1 mg/L (Escherichia coli RF01S in Flask)
  • 2702.8 mg/L (Escherichia coli RF05S-M40 in Flask)
  • 50.5 mg/L (Escherichia coli MG1655/p5C-EC10 in Flask)
  • 88.6 mg/L (Escherichia coli MG1655/p15C-EC10 in Flask)
  • 200.5 mg/L (Escherichia coli MG1655/p20C-BSrib in Flask)
  • 180.3 mg/L (Escherichia coli MG1655/p20C-BS15 in Flask)
  • 192.1 mg/L (Escherichia coli RF01S/p15Trc in Flask)
  • 197.4 mg/L (Escherichia coli RF01S/p15Trc-zg in Flask)
  • 228.4 mg/L (Escherichia coli RF01S/p15Trc-zgp in Flask)
  • 31.9 mg/g of sustrate (Escherichia coli RF01S/p15Trc-zgp in Flask)
  • 394.8 mg/L (Escherichia coli RF02S in Flask)
  • 559.9 mg/L (Escherichia coli RF03S in Flask)
  • 585.2 mg/L (Escherichia coli RF05S in Flask)
  • 1036.1 mg/L (Escherichia coli RF05S-M40 in Flask)
  • 1507.0 mg/L (Escherichia coli RF05S-M40 in Flask)
  Substrate yield
  • 23.0 mg/g of sustrate (Escherichia coli RF01S/p15Trc in Flask)
  • 24.2 mg/g of sustrate (Escherichia coli RF01S/p15Trc-zg in Flask)
  • 39.9 mg/g of sustrate (Escherichia coli RF02S in Flask)
  • 59.9 mg/g of sustrate (Escherichia coli RF03S in Flask)
  • 59.3 mg/g of sustrate (Escherichia coli RF05S in Flask)
  Specific production rate
  • 5.1 mg/g DCW/h (Escherichia coli RF02S in Flask)
  • 8.2 mg/g DCW/h (Escherichia coli RF03S in Flask)
  • 8.6 mg/g DCW/h (Escherichia coli RF05S in Flask)
• acetate
  Titer
  • 1.5 g/L (Escherichia coli RF05S in Flask)
  • 4.0 g/L (Escherichia coli RF03S in Flask)