Hao, Yanan; Pan, Xuewei; Li, Guomin; You, Jiajia; Zhang, Hengwei; Yan, Sihan; Xu, Meijuan & Rao, Zhiming. Construction of a plasmid-free l-leucine overproducing Escherichia coli strain through reprogramming of the metabolic flux. Biotechnology for Biofuels and Bioproducts. 2023, 16(1).

Strains

• Escherichia coli W3110
• Escherichia coli LEU01
• Escherichia coli LEU02
• Escherichia coli LEU04
• Escherichia coli LEU05
• Escherichia coli LEU06
• Escherichia coli LEU07
• Escherichia coli LEU08
• Escherichia coli LEU09
• Escherichia coli LEU10
• Escherichia coli LEU11
• Escherichia coli LEU12
• Escherichia coli LEU13
• Escherichia coli LEU14
• Escherichia coli LEU15
• Escherichia coli LEU16
• Escherichia coli LEU17
• Escherichia coli LEU18
• Escherichia coli LEU19
• Escherichia coli LEU20
• Escherichia coli LEU21
• Escherichia coli LEU22
• Escherichia coli LEU23
• Escherichia coli LEU24
• Escherichia coli LEU25
• Escherichia coli LEU26
• Escherichia coli LEU27
• Escherichia coli LEU28
• Escherichia coli LEU29
• Escherichia coli LEU30
• Escherichia coli W3110 ΔlacI
• Escherichia coli LEU03

Plasmids

• pTrc99a-leuA^fbr-cgb
• pTrc99a-leuA^fbr-ecj

Metabolite production

• L-leucine
  Titer
  • 0.0 g/L (Escherichia coli W3110 in Flask)
  • 3.0 g/L (Escherichia coli LEU01 in Flask)
  • 3.55 g/L (Escherichia coli LEU02 in Flask)
  • 0.0 g/L (Escherichia coli W3110 ΔlacI in Flask)
  • 1.3 g/L (Escherichia coli LEU03 in Flask)
  • 1.9 g/L (Escherichia coli LEU04 in Flask)
  • 2.1 g/L (Escherichia coli LEU05 in Flask)
  • 4.8 g/L (Escherichia coli LEU06 in Flask)
  • 6.27 g/L (Escherichia coli LEU07 in Flask)
  • 6.44 g/L (Escherichia coli LEU08 in Flask)
  • 6.8 g/L (Escherichia coli LEU09 in Flask)
  • 7.35 g/L (Escherichia coli LEU10 in Flask)
  • 8.0 g/L (Escherichia coli LEU11 in Flask)
  • 8.45 g/L (Escherichia coli LEU12 in Flask)
  • 8.23 g/L (Escherichia coli LEU13 in Flask)
  • 8.74 g/L (Escherichia coli LEU14 in Flask)
  • 10.15 g/L (Escherichia coli LEU15 in Flask)
  • 10.76 g/L (Escherichia coli LEU16 in Flask)
  • 11.8 g/L (Escherichia coli LEU17 in Flask)
  • 11.62 g/L (Escherichia coli LEU18 in Flask)
  • 11.26 g/L (Escherichia coli LEU19 in Flask)
  • 16.1 g/L (Escherichia coli LEU20 in Flask)
  • 16.57 g/L (Escherichia coli LEU21 in Flask)
  • 16.69 g/L (Escherichia coli LEU22 in Flask)
  • 17.15 g/L (Escherichia coli LEU23 in Flask)
  • 12.66 g/L (Escherichia coli LEU26 in Flask)
  • 16.25 g/L (Escherichia coli LEU27 in Flask)
  • 11.15 g/L (Escherichia coli LEU29 in Flask)
  • 13.04 g/L (Escherichia coli LEU30 in Flask)
  • 12.66 g/L (Escherichia coli LEU28 in Flask)
  • 54.6 g/L (Escherichia coli LEU23 in Fed-Batch)
  • 55.0 g/L (Escherichia coli LEU27 in Fed-Batch)
  Substrate yield
  • 0.0 g/g of sustrate (Escherichia coli W3110 in Flask)
  • 0.025 g/g of sustrate (Escherichia coli LEU01 in Flask)
  • 0.03 g/g of sustrate (Escherichia coli LEU02 in Flask)
  • 0.0 g/g of sustrate (Escherichia coli W3110 ΔlacI in Flask)
  • 0.011 g/g of sustrate (Escherichia coli LEU03 in Flask)
  • 0.016 g/g of sustrate (Escherichia coli LEU04 in Flask)
  • 0.018 g/g of sustrate (Escherichia coli LEU05 in Flask)
  • 0.04 g/g of sustrate (Escherichia coli LEU06 in Flask)
  • 0.052 g/g of sustrate (Escherichia coli LEU07 in Flask)
  • 0.054 g/g of sustrate (Escherichia coli LEU08 in Flask)
  • 0.057 g/g of sustrate (Escherichia coli LEU09 in Flask)
  • 0.067 g/g of sustrate (Escherichia coli LEU11 in Flask)
  • 0.07 g/g of sustrate (Escherichia coli LEU12 in Flask)
  • 0.069 g/g of sustrate (Escherichia coli LEU13 in Flask)
  • 0.073 g/g of sustrate (Escherichia coli LEU14 in Flask)
  • 0.085 g/g of sustrate (Escherichia coli LEU15 in Flask)
  • 0.09 g/g of sustrate (Escherichia coli LEU16 in Flask)
  • 0.097 g/g of sustrate (Escherichia coli LEU18 in Flask)
  • 0.098 g/g of sustrate (Escherichia coli LEU17 in Flask)
  • 0.094 g/g of sustrate (Escherichia coli LEU19 in Flask)
  • 0.13 g/g of sustrate (Escherichia coli LEU20 in Flask)
  • 0.14 g/g of sustrate (Escherichia coli LEU21 in Flask)
  • 0.14 g/g of sustrate (Escherichia coli LEU22 in Flask)
  • 0.14 g/g of sustrate (Escherichia coli LEU23 in Flask)
  • 0.2 g/g of sustrate (Escherichia coli LEU27 in Flask)
  • 0.16 g/g of sustrate (Escherichia coli LEU26 in Flask)
  • 0.16 g/g of sustrate (Escherichia coli LEU28 in Flask)
  • 0.14 g/g of sustrate (Escherichia coli LEU29 in Flask)
  • 0.16 g/g of sustrate (Escherichia coli LEU30 in Flask)
  • 0.17 g/g of sustrate (Escherichia coli LEU23 in Fed-Batch)
  • 0.23 g/g of sustrate (Escherichia coli LEU27 in Fed-Batch)
• L-alanine
  Substrate yield
  • 0.061 g/g of sustrate (Escherichia coli LEU10 in Flask)