Liu, Shuang; Kang, Pei; Cui, Zhenzhen; Wang, Zhiwen & Chen, Tao. Increased riboflavin production by knockout of 6-phosphofructokinase I and blocking the Entner–Doudoroff pathway in Escherichia coli. Biotechnology Letters. 2016, 38(8), 1307-1314.

Strains

• Escherichia coli MG1655
• Escherichia coli RF01S
• Escherichia coli RF01T
• Escherichia coli RF03
• Escherichia coli RF03S
• Escherichia coli RF03T
• Escherichia coli RF05S-M40
• Escherichia coli LS01
• Escherichia coli LS02
• Escherichia coli LS01T
• Escherichia coli LS02T

Plasmids

• pLS01
• p20C-EC10

Metabolite production

• riboflavin
  Titer
  • 2.7 g/L (Escherichia coli RF05S-M40 in Flask)
  • 220.0 mg/L (Escherichia coli RF01S in Flask)
  • 563.8 mg/L (Escherichia coli RF03S in Flask)
  • 516.0 mg/L (Escherichia coli RF03T in Flask)
  • 5.8 g/L (Escherichia coli LS02T in Fed-Batch)
  • 667.0 mg/L (Escherichia coli LS02T in Flask)
  • 502.0 mg/L (Escherichia coli RF03T in Flask)
  • 540.0 mg/L (Escherichia coli RF03T in Flask)
  • 570.0 mg/L (Escherichia coli RF03T in Flask)
  • 597.0 mg/L (Escherichia coli RF03T in Flask)
  • 605.0 mg/L (Escherichia coli LS02T in Flask)
  • 10.4 g/L (Escherichia coli LS02T in Fed-Batch)
  • 551.0 mg/L (Escherichia coli LS02T in Flask)
  • 327.0 mg/L (Escherichia coli LS02T in Flask)
  • 296.0 mg/L (Escherichia coli LS02T in Flask)
  Substrate yield
  • 0.13 g/g of sustrate (Escherichia coli RF05S-M40 in Flask)
  • 67.9 mg/g of sustrate (Escherichia coli LS02T in Flask)
  • 50.2 mg/g of sustrate (Escherichia coli RF03T in Flask)
  • 54.1 mg/g of sustrate (Escherichia coli RF03T in Flask)
  • 57.2 mg/g of sustrate (Escherichia coli RF03T in Flask)
  • 59.6 mg/g of sustrate (Escherichia coli RF03T in Flask)
  • 56.8 mg/g of sustrate (Escherichia coli LS02T in Fed-Batch)
  • 61.7 mg/g of sustrate (Escherichia coli LS02T in Flask)
  • 26.8 mg/g of sustrate (Escherichia coli LS02T in Flask)
  • 53.4 mg/g of sustrate (Escherichia coli LS02T in Flask)
  • 31.8 mg/g of sustrate (Escherichia coli LS02T in Flask)
  Specific production rate
  • 7.8 mg/g DCW/h (Escherichia coli LS02T in Flask)
  • 5.5 mg/g DCW/h (Escherichia coli RF03T in Flask)
  • 5.7 mg/g DCW/h (Escherichia coli RF03T in Flask)
  • 5.9 mg/g DCW/h (Escherichia coli RF03T in Flask)
  • 5.8 mg/g DCW/h (Escherichia coli RF03T in Flask)
  • 2.9 mg/g DCW/h (Escherichia coli LS02T in Flask)
  • 6.1 mg/g DCW/h (Escherichia coli LS02T in Flask)
  • 3.4 mg/g DCW/h (Escherichia coli LS02T in Flask)
  • 6.7 mg/g of sustrate (Escherichia coli LS02T in Flask)