Hou, Minglei; Gao, Shengqi; Wu, Jing; Chen, Sheng & Zhang, Kang. Metabolic engineering of Escherichia coli to enhance L-tryptophan biosynthesis. Systems Microbiology and Biomanufacturing. 2025.

Strains

• Escherichia coli JM109
• Escherichia coli W3110
• Escherichia coli TS
• Escherichia coli TS-0
• Escherichia coli TS-1
• Escherichia coli TS-1A
• Escherichia coli ZH-1
• Escherichia coli ZH-2
• Escherichia coli ZH-3
• Escherichia coli ZH-4
• Escherichia coli HL-1
• Escherichia coli HL-2
• Escherichia coli TS-2
• Escherichia coli TS-3
• Escherichia coli TS-1B
• Escherichia coli HL-3
• Escherichia coli HL-4

Plasmids

• pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA
• pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A
• pBR322-P_tac-aroG^S211F-P_tac-trpE^S40F-trpABCD-serA^H344A/N364A
• pBR322-P_tac-aroG^S211F-P_tac−trpE^A63V-trpABCD-serA^H344A/N364A

Metabolite production

• L-tryptophan
  Substrate yield
  • 0.135 g/g of sustrate (Escherichia coli TS-1B in Flask)
  • 0.18 g/g of sustrate (Escherichia coli ZH-3 in Fed-Batch)
  • 0.086 g/g of sustrate (Escherichia coli TS-1A in Flask)
  • 0.087 g/g of sustrate (Escherichia coli TS-1 in Flask)
  • 0.056 g/g of sustrate (Escherichia coli ZH-1 in Flask)
  • 0.082 g/g of sustrate (Escherichia coli ZH-2 in Flask)
  • 0.092 g/g of sustrate (Escherichia coli ZH-3 in Flask)
  • 0.129 g/g of sustrate (Escherichia coli ZH-4 in Flask)
  • 0.1 g/g of sustrate (Escherichia coli TS in Flask)
  Titer
  • 43.0 g/L (Escherichia coli ZH-3 in Fed-Batch)
  • 5.99 g/L (Escherichia coli TS in Flask)
  • 5.05 g/L (Escherichia coli TS-1 in Flask)
  • 5.2 g/L (Escherichia coli TS-2 in Flask)
  • 2.3 g/L (Escherichia coli TS-3 in Flask)
  • 3.27 g/L (Escherichia coli TS-1A in Flask)
  • 4.32 g/L (Escherichia coli TS-1B in Flask)
  • 3.09 g/L (Escherichia coli ZH-1 in Flask)
  • 4.47 g/L (Escherichia coli ZH-2 in Flask)
  • 5.0 g/L (Escherichia coli ZH-3 in Flask)
  • 5.33 g/L (Escherichia coli ZH-4 in Flask)
  • 5.43 g/L (Escherichia coli TS in Flask)
  • 5.3 g/L (Escherichia coli ZH-3 in Flask)
  • 5.4 g/L (Escherichia coli HL-1 in Flask)
  • 5.1 g/L (Escherichia coli ZH-3 in Flask)
  • 5.08 g/L (Escherichia coli HL-2 in Flask)
  • 5.39 g/L (Escherichia coli HL-3 in Flask)
  • 5.66 g/L (Escherichia coli HL-4 in Flask)
  Volumetric productivity
  • 1.23 g/L/h (Escherichia coli ZH-3 in Fed-Batch)