Ping, Jurong; Wang, Lian; Qin, Zhijie; Zhou, Zhemin & Zhou, Jingwen. Synergetic engineering of Escherichia coli for efficient production of l-tyrosine. Synthetic and Systems Biotechnology. 2023, 8(4), 724-731.

Strains

• Escherichia coli WSH-Z06
• Escherichia coli HGA1
• Escherichia coli HGA2
• Escherichia coli HGA3
• Escherichia coli HGA4
• Escherichia coli HGA5
• Escherichia coli HGA6
• Escherichia coli HGB1
• Escherichia coli HGB2
• Escherichia coli HGB3
• Escherichia coli HGB4
• Escherichia coli HGB5
• Escherichia coli HGB6
• Escherichia coli HGC1
• Escherichia coli HGC2
• Escherichia coli HGC3
• Escherichia coli HGC4
• Escherichia coli HGD1
• Escherichia coli HGD2
• Escherichia coli HGD3
• Escherichia coli HGD (M9)
• Escherichia coli WSH-Z06 (pAP22)
• Escherichia coli WSH-Z06 (pAP21)
• Escherichia coli WSH-Z06 (pAP13)
• Escherichia coli WSH-Z06 (pAP12)
• Escherichia coli WSH-Z06 (pAP11)
• Escherichia coli WSH-Z06 (pAP01)
• Escherichia coli WSH-Z06 (pAP01) ΔldhA
• Escherichia coli WSH-Z06 (pAP01) ΔpflB
• Escherichia coli WSH-Z06 (pAP01) ΔadhE
• Escherichia coli WSH-Z06 (pAP01) ΔtrpE
• Escherichia coli WSH-Z06 (pAP01) ΔpheA
• Escherichia coli WSH-Z06 (pAP01) ΔtyrR
• Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA
• Escherichia coli WSH-Z06 (pAP01) ΔtyrR,trpE
• Escherichia coli WSH-Z06 (pAP01) ΔtyrR,adhE
• Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pflB
• Escherichia coli WSH-Z06 (pAP01) ΔtyrR,ldhA
• Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA,trpE
• Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA,adhE
• Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA,pflB
• Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA,ldhA

Plasmids

• pAP-B03
• pCDF-aroG^fbr-tyrA^fbr
• pAP32
• pAP31
• pAP13
• pAP12
• pAP11
• pAP01
• pAP33
• pAP22
• pAP21

Metabolite production

• L-tyrosine
  Titer
  • 4.22 g/L (Escherichia coli HGA1 in Flask)
  • 6.17 g/L (Escherichia coli HGC4 in Flask)
  • 50.2 g/L (Escherichia coli HGC4 in Fed-Batch)
  • 7.11 g/L (Escherichia coli HGD (M9) in Flask)
  • 80.5 g/L (Escherichia coli HGD (M9) in Fed-Batch)
  • 92.8 g/L (Escherichia coli HGD (M9) in Fed-Batch)
  • 1.5 g/L (Escherichia coli WSH-Z06 (pAP12) in Flask)
  • 1.8 g/L (Escherichia coli WSH-Z06 (pAP11) in Flask)
  • 1.1 g/L (Escherichia coli WSH-Z06 (pAP22) in Flask)
  • 1.4 g/L (Escherichia coli WSH-Z06 (pAP21) in Flask)
  • 0.8 g/L (Escherichia coli WSH-Z06 (pAP13) in Flask)
  • 1.2 g/L (Escherichia coli WSH-Z06 (pAP01) in Flask)
  • 1.47 g/L (Escherichia coli WSH-Z06 (pAP01) ΔldhA in Flask)
  • 1.29 g/L (Escherichia coli WSH-Z06 (pAP01) ΔpflB in Flask)
  • 1.67 g/L (Escherichia coli WSH-Z06 (pAP01) ΔadhE in Flask)
  • 1.34 g/L (Escherichia coli WSH-Z06 (pAP01) ΔtrpE in Flask)
  • 1.47 g/L (Escherichia coli WSH-Z06 (pAP01) ΔpheA in Flask)
  • 2.13 g/L (Escherichia coli WSH-Z06 (pAP01) ΔtyrR in Flask)
  • 3.2 g/L (Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA in Flask)
  • 1.98 g/L (Escherichia coli WSH-Z06 (pAP01) ΔtyrR,trpE in Flask)
  • 3.08 g/L (Escherichia coli WSH-Z06 (pAP01) ΔtyrR,adhE in Flask)
  • 3.68 g/L (Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pflB in Flask)
  • 2.27 g/L (Escherichia coli WSH-Z06 (pAP01) ΔtyrR,ldhA in Flask)
  • 1.8 g/L (Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA,trpE in Flask)
  • 2.19 g/L (Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA,adhE in Flask)
  • 2.79 g/L (Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA,pflB in Flask)
  • 3.18 g/L (Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA,ldhA in Flask)
  • 3.85 g/L (Escherichia coli HGA2 in Flask)
  • 3.49 g/L (Escherichia coli HGA3 in Flask)
  • 3.7 g/L (Escherichia coli HGA4 in Flask)
  • 3.2 g/L (Escherichia coli HGA5 in Flask)
  • 3.61 g/L (Escherichia coli HGA6 in Flask)
  Substrate yield
  • 0.278 g/g of sustrate (Escherichia coli HGD (M9) in Fed-Batch)
  • 0.266 g/g of sustrate (Escherichia coli HGD (M9) in Fed-Batch)
• acetate
  Titer
  • 12.4 g/L (Escherichia coli HGC4 in Fed-Batch)
  • 9.0 g/L (Escherichia coli HGD (M9) in Fed-Batch)