Siyu Zhao, Tangen Shi, Liangwen Li, Zhichao Chen, Changgeng Li, Zichen Yu, Pengjie Sun & Qingyang Xu (2024). The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine. Microbial Cell Factories.

Strains

• Escherichia coli Y2-1
• Escherichia coli Y2-2
• Escherichia coli Y2-3
• Escherichia coli Y2-4
• Escherichia coli Y3-1
• Escherichia coli Y3-2
• Escherichia coli Y3-3
• Escherichia coli Y3-4
• Escherichia coli Y4-1
• Escherichia coli Y4-2
• Escherichia coli Y4-3
• Escherichia coli Y5-1
• Escherichia coli Y5-3
• Escherichia coli Y6-1
• Escherichia coli Y6-2
• Escherichia coli Y6-3
• Escherichia coli Y6-4
• Escherichia coli Y6-6
• Escherichia coli Y6-7
• Escherichia coli HX1
• Escherichia coli HX2
• Escherichia coli HX3-1
• Escherichia coli HX3-2
• Escherichia coli HX4-1
• Escherichia coli HX4-2
• Escherichia coli HX1-1
• Escherichia coli Y1-2
• Escherichia coli Y5-2
• Escherichia coli Y5-4A
• Escherichia coli Y5-4B
• Escherichia coli Y5-4C
• Escherichia coli Y5-4D
• Escherichia coli Y6
• Escherichia coli Y6-5
• Escherichia coli HX5
• Escherichia coli DH5α
• Escherichia coli W3110

Metabolite production

• hypoxanthine
  Titer
  • 0.49 g/L (Escherichia coli HX1-1 in Flask)
  • 0.82 g/L (Escherichia coli Y1-2 in Flask)
  • 1.38 g/L (Escherichia coli Y1-2 in Flask)
  • 3.43 g/L (Escherichia coli Y5-1 in Flask)
  • 2.67 g/L (Escherichia coli Y5-4A in Flask)
  • 3.25 g/L (Escherichia coli Y5-4B in Flask)
  • 3.19 g/L (Escherichia coli Y5-4C in Flask)
  • 3.23 g/L (Escherichia coli Y5-4D in Flask)
  • 0.1 g/L (Escherichia coli HX2 in Flask)
  • 50.0 mg/L (Escherichia coli HX1 in Flask)
  • 0.2 g/L (Escherichia coli HX3-1 in Flask)
  • 0.32 g/L (Escherichia coli HX3-2 in Flask)
  • 0.41 g/L (Escherichia coli HX4-1 in Flask)
  • 0.53 g/L (Escherichia coli HX4-2 in Flask)
  • 0.0 g/L (Escherichia coli W3110 in Flask)
  • 0.94 g/L (Escherichia coli HX5 in Flask)
  • 0.6 g/L (Escherichia coli Y2-1 in Flask)
  • 1.32 g/L (Escherichia coli Y2-2 in Flask)
  • 1.11 g/L (Escherichia coli Y2-3 in Flask)
  • 1.18 g/L (Escherichia coli Y2-4 in Flask)
  • 0.71 g/L (Escherichia coli Y3-1 in Flask)
  • 1.25 g/L (Escherichia coli Y3-2 in Flask)
  • 1.1 g/L (Escherichia coli Y3-3 in Flask)
  • 1.67 g/L (Escherichia coli Y3-4 in Flask)
  • 1.95 g/L (Escherichia coli Y4-1 in Flask)
  • 2.27 g/L (Escherichia coli Y4-2 in Flask)
  • 2.89 g/L (Escherichia coli Y4-3 in Flask)
  • 2.11 g/L (Escherichia coli Y5-1 in Flask)
  • 3.3 g/L (Escherichia coli Y6 in Flask)
  • 3.61 g/L (Escherichia coli Y6-1 in Flask)
  • 3.21 g/L (Escherichia coli Y6-3 in Flask)
  • 3.94 g/L (Escherichia coli Y6-7 in Flask)
  • 4.02 g/L (Escherichia coli Y6-6 in Flask)
  • 4.11 g/L (Escherichia coli Y6-5 in Flask)
  • 3.67 g/L (Escherichia coli Y6-4 in Flask)
  • 3.84 g/L (Escherichia coli Y6-2 in Flask)
  • 30.6 g/L (Escherichia coli Y6-5 in Fed-Batch)
  Substrate yield
  • 0.035 g/g of sustrate (Escherichia coli Y3-4 in Flask)
  • 0.03 g/g of sustrate (Escherichia coli Y4-1 in Flask)
  • 0.046 g/g of sustrate (Escherichia coli Y4-2 in Flask)
  • 0.06 g/g of sustrate (Escherichia coli Y4-3 in Flask)
  • 0.065 g/g of sustrate (Escherichia coli Y6 in Flask)
  • 0.071 g/g of sustrate (Escherichia coli Y6-1 in Flask)
  • 0.06 g/g of sustrate (Escherichia coli Y6-2 in Flask)
  • 0.057 g/g of sustrate (Escherichia coli Y6-3 in Flask)
  • 0.074 g/g of sustrate (Escherichia coli Y6-5 in Flask)
  • 0.067 g/g of sustrate (Escherichia coli Y6-7 in Flask)
  • 0.07 g/g of sustrate (Escherichia coli Y6-6 in Flask)
  • 0.074 g/g of sustrate (Escherichia coli Y6-5 in Flask)
  • 0.062 g/g of sustrate (Escherichia coli Y6-4 in Flask)
• AMP
  Titer
  • 0.19 g/L (Escherichia coli Y1-2 in Flask)
  • 0.0 g/L (Escherichia coli HX1-1 in Flask)
  • 0.17 g/L (Escherichia coli Y1-2 in Flask)
  • 0.34 g/L (Escherichia coli HX5 in Flask)
  • 0.53 g/L (Escherichia coli Y2-1 in Flask)
  • 0.16 g/L (Escherichia coli Y2-2 in Flask)
  • 0.28 g/L (Escherichia coli Y2-3 in Flask)
  • 0.25 g/L (Escherichia coli Y2-4 in Flask)
  • 0.45 g/L (Escherichia coli Y3-1 in Flask)
  • 0.21 g/L (Escherichia coli Y3-2 in Flask)
  • 0.27 g/L (Escherichia coli Y3-3 in Flask)
  • 0.11 g/L (Escherichia coli Y3-4 in Flask)
  • 0.45 g/L (Escherichia coli Y4-1 in Flask)
  • 0.0 g/L (Escherichia coli Y4-2 in Flask)
  • 0.0 g/L (Escherichia coli Y4-3 in Flask)
• xanthine
  Titer
  • 0.23 g/L (Escherichia coli HX5 in Flask)
  • 0.33 g/L (Escherichia coli HX1-1 in Flask)
  • 0.11 g/L (Escherichia coli Y1-2 in Flask)
  • 0.2 g/L (Escherichia coli Y1-2 in Flask)
  • 0.21 g/L (Escherichia coli Y2-1 in Flask)
  • 0.23 g/L (Escherichia coli Y2-2 in Flask)
  • 0.23 g/L (Escherichia coli Y2-3 in Flask)
  • 0.23 g/L (Escherichia coli Y2-4 in Flask)
  • 0.23 g/L (Escherichia coli Y3-1 in Flask)
  • 0.23 g/L (Escherichia coli Y3-2 in Flask)
  • 0.21 g/L (Escherichia coli Y3-3 in Flask)
  • 0.23 g/L (Escherichia coli Y3-4 in Flask)
  • 0.37 g/L (Escherichia coli Y4-1 in Flask)
  • 0.22 g/L (Escherichia coli Y4-2 in Flask)
  • 0.2 g/L (Escherichia coli Y4-3 in Flask)
  • 0.0 g/L (Escherichia coli Y5-4D in Flask)
  • 0.0 g/L (Escherichia coli Y5-4C in Flask)
  • 0.0 g/L (Escherichia coli Y5-4B in Flask)
  • 0.1 g/L (Escherichia coli Y5-4A in Flask)
  • 0.0 g/L (Escherichia coli Y5-1 in Flask)
  • 0.0 g/L (Escherichia coli Y5-1 in Flask)
• guanine
  Titer
  • 0.15 g/L (Escherichia coli HX5 in Flask)
  • 0.25 g/L (Escherichia coli HX1-1 in Flask)
  • 0.07 g/L (Escherichia coli Y1-2 in Flask)
  • 0.13 g/L (Escherichia coli Y1-2 in Flask)
  • 0.16 g/L (Escherichia coli Y2-1 in Flask)
  • 0.15 g/L (Escherichia coli Y2-2 in Flask)
  • 0.15 g/L (Escherichia coli Y2-3 in Flask)
  • 0.15 g/L (Escherichia coli Y2-4 in Flask)
  • 0.15 g/L (Escherichia coli Y3-1 in Flask)
  • 0.15 g/L (Escherichia coli Y3-2 in Flask)
  • 0.15 g/L (Escherichia coli Y3-3 in Flask)
  • 0.15 g/L (Escherichia coli Y3-4 in Flask)
  • 0.26 g/L (Escherichia coli Y4-1 in Flask)
  • 0.11 g/L (Escherichia coli Y4-2 in Flask)
  • 0.1 g/L (Escherichia coli Y4-3 in Flask)
  • 0.0 g/L (Escherichia coli Y5-4D in Flask)
  • 0.0 g/L (Escherichia coli Y5-4C in Flask)
  • 0.0 g/L (Escherichia coli Y5-4B in Flask)
  • 0.0 g/L (Escherichia coli Y5-4A in Flask)
  • 0.0 g/L (Escherichia coli Y5-1 in Flask)
  • 0.0 g/L (Escherichia coli Y5-1 in Flask)
• adenine
  Titer
  • 0.0 g/L (Escherichia coli HX5 in Flask)
  • 0.24 g/L (Escherichia coli Y2-1 in Flask)
  • 0.0 g/L (Escherichia coli Y2-2 in Flask)
  • 0.0 g/L (Escherichia coli Y2-3 in Flask)
  • 0.0 g/L (Escherichia coli Y2-4 in Flask)
  • 0.17 g/L (Escherichia coli Y3-1 in Flask)
  • 0.0 g/L (Escherichia coli Y3-2 in Flask)
  • 0.0 g/L (Escherichia coli Y3-3 in Flask)